Journal: Frontiers in Plant Science

Article Title: Overexpression of CsCaM3 Improves High Temperature Tolerance in Cucumber

doi: 10.3389/fpls.2018.00797

Figure Lengend Snippet: Sequence alignment and phylogenetic tree of CsCaM3 . Alignment analysis of the inferred CsCaM3 protein sequence with other inferred plant calmodulin proteins: CsCaM from Cucumis sativus, CmCaM from Cucumis melo, AtCaM3 from Arabidopsis thaliana, JrCaM7 from Juglans regia, StCaM from Solanum tuberosum, NtCaM7 from Nicotiana tabacum, BoCaM from Brassica oleracea , and SlCaM3 from Solanum lycopersicum . The numbers indicate branch lengths.

Article Snippet: Images were observed with a fluorescent microscope (Olympus BX41; Olympus Corporation, Tokyo, Japan) under blue light excitation (460–495 nm) and emission at 510 nm and analyzed using a photographic apparatus (Olympus CamediaC-5060; Olympus, Tokyo, Japan) and analySIS software (Soft Imaging Solutions GmbH, Münster, Germany).

Techniques: Sequencing

Journal: Frontiers in Plant Science

Article Title: Overexpression of CsCaM3 Improves High Temperature Tolerance in Cucumber

doi: 10.3389/fpls.2018.00797

Figure Lengend Snippet: Analysis of expression characterization of CsCaM3 and in situ hybridization. (A) The expression of CsCaM3 in stem, leaf, and root tissues from cucumber inbred line “02-8.” (B) The expression of CsCaM3 in leaf tissue after 5 μM ABA treatment at different times. (C) The expression of CsCaM3 in leaf tissue after 43°C HT treatment at different times. (D) The expression of CsCaM3 in leaf tissue after 100 μM SA treatment at different times. (E,F) In situ hybridization of CsCaM 3 in leaf tissue in wild-type cucumber control plants and under 43°C HT treatments for 6 h, respectively. Scale bars = 100 μm. The arrows indicate hybridization signals. (G,H) In situ hybridization of CsCaM3 in root tissue in wild-type cucumber control plants and under 43°C HT treatment for 6 h, respectively. ∗ P < 0.05 and ∗∗ P < 0.01 in comparisons of each time points. Scale bars = 50 μm. Each treatment was repeated three times.

Article Snippet: Images were observed with a fluorescent microscope (Olympus BX41; Olympus Corporation, Tokyo, Japan) under blue light excitation (460–495 nm) and emission at 510 nm and analyzed using a photographic apparatus (Olympus CamediaC-5060; Olympus, Tokyo, Japan) and analySIS software (Soft Imaging Solutions GmbH, Münster, Germany).

Techniques: Expressing, In Situ Hybridization, Control, Hybridization